Scientist at Bolt Threads
Emeryville, CA, US
At Bolt Threads, we believe that answers to our most vexing problems can be found in nature. Every day we’re inspired by the amazing materials we work with, and driven by the desire to turn these materials into incredible products. We are a venture backed, idea driven company, led by world-class scientific and engineering talent, as well as experienced executives from the technology and apparel industries.

Scientist – Host Development

You like to tinker with microbes. You have worked with a variety of protein expression and purification systems. You can leverage your strong understanding of physiology and cell biology in order to coerce cells into doing your bidding. You enjoy the challenges of both building up biological systems from scratch, as well as fine tuning them into efficient production hosts.

Bolt Threads is looking for a highly motivated and productive scientist to support the development of novel recombinant protein materials. You are an execution-oriented scientist with superb bench and analytical skills. You are excited by challenging problems, master new material quickly, and play nicely with others. You will be working closely with the strain engineering, fermentation, and materials development teams to rapidly assess the potential of new hosts and novel materials.

The Position

- Evaluate and/or develop hosts (both eukaryotic and prokaryotic) for industrial heterologous protein expression and purification
- Aid in development of non-chromatographic bench-scale processes for protein isolation
- Collaborate with strain engineering, fermentation, and materials development teams to rapidly prototype new protein-based materials
- Conceive, design, execute, analyze, document, and communicate experiments and processes

Requirements

- PhD in Molecular Biology, Microbiology, Biological Engineering, Biochemistry, or related field
- Demonstrated expertise with molecular cloning and genome editing
- Detailed understanding of heterologous protein expression systems in two or more types of microbial hosts (e.g., gram-positive bacteria, gram-negative bacteria, yeast, filamentous fungi)
- Experience with protein purification, preferably including non-chromatographic approaches such as cell fractionation, inclusion body preparation, and three-phase partitioning
- Understanding of bioreactor operations and experience analyzing bioreactor data a plus
- Excellent written and verbal communication
- Ability to lift and manipulate bottles of liquid up to 4L, and work standing up 60% of your workday